Precision Measurement of Vector Copy Number per Transduced Cell and Genome Editing with Simultaneous Immuno-phenotype for ATMP Characterization
Time: 12:15 pm
day: Day 1 Track B- AM
Details:
- Explore the challenges of conventional assays for characterizing cell-based therapies, including: lack of resolution to truly reflect sample composition, population averages, and laborious time consuming workflows
- Demonstrate the advantages of single-cell DNA sequencing in identifying transduced versus non-transduced cells with exceptional accuracy and precision, as well as measure the single-cell level vector copy number (VCN) for populations of thousands of cells
- Application of single-cell sequencing to analyze gene editing outcomes, such as zygosity, co-occurrence, off-target edits, and immunophenotype, while eliminating bioinformatic labor
