Single-Cell Multi-omic Assessment of Gene Editing Outcomes, Toxicity, & VCN for High Resolution Characterization of Cell Therapies
Time: 11:45 am
day: Track A - Cell Based Assay (Day Two - Morning)
Details:
- Despite advancements in cell therapy engineering, conventional methods for measuring gene transfer and gene editing techniques lack the resolution and representation to truly reflect sample composition and either report a population average (bulk) or involve laborious and time-consuming clonal outgrowth.
- Mission Bio has developed an end-to-end solution from panel design to data analysis for single-cell targeted DNA sequencing to interrogate transgenes to confirm the distribution of vector copy number (VCN).
- Additionally, the solution simultaneously measures the co-occurrence and zygosity of on-target edits, off-target edits, translocations between predicted edit sites, as well as the genomic CNV landscape (including focal CNV) in over thousands of cells in parallel to reveal potential genotoxicity events in a single-cell context.
