Utilization of Digital PCR for Quantification of CAR T Cell Products

Time: 12:30 pm
day: Day One


• Digital PCR (dPCR) has many advantages compared to qPCR for quantification
of CAR T cell products including simplicity, reproducibility, lower limit of detection,
and absolute quantification
• Describe the development of a dPCR assay to specifically detect and quantify the
novel bicistronic CD19xCD22 CAR T cell product
• Demonstrate that digital PCR strategies can be utilized for accurate, precise, and
reproducible quantification of unique CAR transgenes and Vector Copy Number
(VCN) measurements